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Curve Fitting

The Curve Fitting app within the Ganymede platform provides a comprehensive solution for analyzing dose-response, standard curves, and binding assays. This specialized application automates regression analysis while enabling interactive outlier management and maintaining complete audit trails. The app supports various regression models including linear, 4-parameter logistic (4PL), 5-parameter logistic (5PL), and custom equations, enabling standardized curve fitting workflows across your organization.

Overview

As part of the Ganymede data platform, the Curve Fitting app transforms how laboratories analyze concentration-response data by providing interactive visualization, automated regression fitting, and outlier management in a single unified interface. The app eliminates manual curve fitting in spreadsheets while ensuring consistency across different users and experiments. Users can iteratively refine fits, document exclusion decisions, and generate validated results while maintaining full traceability for regulatory compliance.

Common Use Cases

The Curve Fitting app excels in various analytical scenarios:

  • Dose-Response Analysis — Determine IC50/EC50 values for drug discovery
  • Antibody Titer Determination — Quantify antibody concentrations from serial dilutions
  • Protein Quantification — Generate standard curves for BCA, Bradford, and other assays
  • Receptor-Ligand Binding — Analyze binding kinetics and affinity constants
  • ELISA Standard Curves — Fit calibration curves for immunoassays
  • Cell Viability Assays — Calculate growth inhibition and cytotoxicity
  • Bioassay Potency — Determine relative potency for lot release
  • Enzyme Kinetics — Fit Michaelis-Menten and inhibition curves

The features shown below provide a representative set of capabilities available in the Curve Fitting app. Your implementation will be tailored to your specific assay types, regression models, and analytical requirements.

Representative Analysis Workflow

The Curve Fitting app follows a logical workflow that guides users through data analysis:

StepActionDescription
1️⃣Data ImportLoad experimental data from Ganymede's data lake with automatic parsing
2️⃣Model SelectionChoose appropriate regression model (linear, 4PL, 5PL, custom)
3️⃣Initial FitGenerate curve with calculated parameters and goodness-of-fit metrics
4️⃣Interactive RefinementClick data points to exclude outliers with real-time updates
5️⃣DocumentationAnnotate exclusion decisions for complete audit trail
6️⃣Results ExportSave validated curves and parameters back to Ganymede
note

The workflow steps shown are representative. Your implementation will be customized to integrate seamlessly with your existing processes, performing only the relevant steps within the Ganymede app while maintaining your established laboratory workflows.

Key Features

Data Loading and Visualization

Efficiently load and visualize concentration-response data with comprehensive metadata:

Curve Fitting initial data loading screen with instructions

The app provides clear instructions and intuitive controls for data loading and analysis setup.

 

  • Multiple data sources — Import from plates, tubes, or custom formats
  • Automatic plotting — Instant visualization of concentration-response relationships
  • Metadata integration — Display sample names, conditions, and experimental details
  • Interactive tooltips — Hover to view detailed information for each data point

Advanced Curve Fitting

Generate regression fits with multiple model options and real-time parameter calculation:

4PL curve fit showing dose-response relationship with calculated parameters

The app automatically fits data to the selected regression model, displaying the curve with confidence bands and calculated parameters.

 

Supported Regression Models

Model TypeParametersCommon Applications
LinearSlope, InterceptStandard curves with narrow range
4PL (Four Parameter Logistic)Min, Max, IC50, Hill SlopeDose-response, ELISA
5PL (Five Parameter Logistic)Min, Max, IC50, Hill Slope, AsymmetryAsymmetric curves, immunoassays
Hill EquationVmax, Km, nEnzyme kinetics, cooperativity
ExponentialA, kGrowth curves, decay kinetics
Custom EquationsUser-definedSpecialized assay requirements

Interactive Outlier Management

Identify and exclude outliers with full documentation and real-time fit updates:

Curve fit with outliers marked in red, showing interactive point exclusion

Click any data point to toggle its inclusion in the fit. Excluded points are marked in red (configurable for accessibility).

 

  • Click-to-exclude — Simple point selection for outlier identification
  • Visual feedback — Color and shape changes for excluded points
  • Real-time updates — Instant recalculation of fit parameters
  • Undo/redo support — Easily revert exclusion decisions
  • Batch operations — Exclude multiple points based on criteria

Results Validation and Metrics

Comprehensive metrics display with annotation capabilities for excluded points:

Metrics table showing fit parameters, R-squared, and excluded points with annotation fields

Document reasons for point exclusion and review all fit metrics in a comprehensive summary table.

 

Quality Metrics

MetricDescriptionAcceptance Criteria
Coefficient of determinationTypically >0.95
IC50/EC50Half-maximal concentrationWithin expected range
Hill SlopeCurve steepness-3 to -0.3 for typical assays
95% CIConfidence intervalsNarrow bands indicate good fit
ResidualsDifference from fitRandom distribution expected
Z-factorAssay quality metric>0.5 for screening assays

Advanced Analysis Features

Additional capabilities for specialized analyses:

  • Parallelism testing — Compare curves for similarity
  • Relative potency — Calculate potency ratios between samples
  • Linear range detection — Identify optimal quantification range
  • Weighting options — 1/Y, 1/Y², or custom weighting schemes
  • Constraint setting — Fix parameters or set bounds
  • Global fitting — Fit multiple curves with shared parameters

Data Types Analyzed

The Curve Fitting app processes various types of concentration-response data:

Raw Measurement Data

  • Absorbance values — Colorimetric assay readouts
  • Fluorescence intensities — Fluorescent assay signals
  • Luminescence counts — Reporter gene and viability assays
  • Concentration series — Dilution schemes and stock concentrations
  • Replicate measurements — Technical and biological replicates

Calculated Parameters

  • IC50/EC50 values — Half-maximal inhibitory/effective concentrations
  • Slope parameters — Hill slope, linear slope coefficients
  • Dynamic range — Minimum and maximum asymptotes
  • Confidence intervals — Parameter uncertainty estimates
  • Goodness-of-fit — R², adjusted R², RMSE

Quality Control Data

  • Outlier flags — Automated and manual exclusions
  • Exclusion reasons — User annotations for audit trail
  • Replicate statistics — CV%, standard deviations
  • Assay controls — Positive/negative control values

Data Integration

The Curve Fitting app seamlessly integrates with Ganymede's data infrastructure:

Data Management Features

  • Direct data lake connection — Access all assay data stored in Ganymede tables
  • Automatic data parsing — Extract concentration-response pairs and metadata
  • Template management — Save and reuse analysis configurations
  • Batch processing — Analyze multiple curves simultaneously
  • Version control — Track analysis iterations and parameter changes
  • Audit trail maintenance — Complete record of exclusions and decisions

Export Capabilities

Export TypeFormatsContents
Raw DataCSV, ExcelOriginal measurements with metadata
Fitted CurvesCSV, Excel, JSONParameters, fitted values, residuals
ReportsPDF, HTMLComplete analysis with figures and annotations
FiguresPNG, SVG, PDFPublication-ready plots
Analysis LogJSON, CSVAudit trail of all user actions

Customization Options

The Curve Fitting app can be readily configured to suit specific assay requirements:

  • Custom regression models — Add proprietary equations
  • Assay-specific validations — Define acceptance criteria
  • Automated outlier detection — Statistical methods for flagging
  • Report templates — Customized output formats
  • Integration workflows — Connect to LIMS or ELN systems
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The Implementation team can help customize:

  • Standard and baseline definitions
  • Linear range determination algorithms
  • Specialized curve fitting models
  • Validation rules and acceptance criteria

Workflow Benefits

Implementing the Curve Fitting app in your laboratory workflows provides significant advantages:

  • Reduced analysis time — Automate curve fitting that typically requires manual work
  • Improved consistency — Standardize regression methods across all users
  • Enhanced traceability — Document all exclusion decisions with reasons
  • Better collaboration — Share validated curves across teams
  • Regulatory compliance — Maintain complete audit trails for submissions
  • Increased accuracy — Eliminate manual transcription and calculation errors

Learn More

The Curve Fitting app transforms concentration-response analysis from manual spreadsheet work into an efficient, validated workflow. To explore how the Curve Fitting app can be tailored to your specific assay requirements, contact your Ganymede representative or our sales team to learn more.